FastQCFastQC Report
Fri 29 Apr 2016
ERR1042522.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042522.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570223
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT38470.6746483393339098No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG19730.34600498401502566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11650.2043060346566168No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG10510.1843138561580294No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG8870.15555317831795631No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT7990.14012061947694146No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTA7440.1304752702013072No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCA7370.1292476802934992No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA7060.12381121070177807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.12310973075445922No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC6890.12082992092567295No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG6740.11819937112322722No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA6700.11749789117590838No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA6260.10978161175540097No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC6070.1064495820056364No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAA5940.10416977217685011No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG5830.10224070232172325No Hit
GTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT5820.10206533233489354No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG5740.10066237244025582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAG250.00549404929.65
CGGATCA250.00549404929.636
GGCATAC250.00549404929.61
GTTATAC250.00549404929.63
TGTATAC405.9349622E-527.753
TAGTCCG358.8638917E-426.4285725
GCGCAAG7700.024.5064931
CGCAAGA7550.024.258282
ACGGACC7650.024.1830068
CAAGACG7600.024.0986844
AAGACGG7700.024.0259725
CGGACCA7800.023.7179479
AGACGGA8100.023.524696
TAGACTC400.001930000723.1255
GTTAGAG400.001930000723.1253
GACGGAC8150.022.926387
TATACAG652.6789094E-622.769235
CATTTGC8100.022.6111125
TCGCGTA4050.022.3827159
GCAAGAC8350.022.3772453
Produced by FastQC (version 0.11.5)