FastQCFastQC Report
Fri 29 Apr 2016
ERR1042521.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042521.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044566
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT59030.19388641927946382No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC37720.12389286354771091No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37000.12152799446620635No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG33360.10957226744304444No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG32570.10697748053417137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22450.022.7438741
TCGTTTA7650.018.13725530
AAGACGG14000.018.1035735
CGCAAGA13750.018.029092
ATGGTCG7900.017.79746836
TGGTCGG7900.017.79746837
GCGCAAG14750.017.3084741
ACGGACC14150.017.2579528
CGTTTAT8150.017.25153531
CAAGACG14050.017.249114
TACCGTC6350.016.8976387
GTATCAA29800.016.7617452
AGACGGA15450.016.045316
GACGGAC15000.016.0333357
CGAACGT2800.015.8571424
CGGACCA15200.015.8223699
TCGCGTA9650.015.7202079
ATACCGT7400.015.5000016
CCGGTCG8900.015.38202320
TATGGTC9150.015.36612135
Produced by FastQC (version 0.11.5)