FastQCFastQC Report
Fri 29 Apr 2016
ERR1042520.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042520.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1860385
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT123040.6613684801801778No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT102980.5535413368738191No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80120.43066354544892593No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50820.27316926335140307No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24090.12948932613410666No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21070.1132561270919729No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20580.11062226367122933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24950.029.066131
CGATCGC902.1536725E-618.519
GTATCAA41800.017.3050232
TAACGCC2300.016.8913044
AATCGTC1352.2217137E-816.44444528
TATACTG3300.016.2575765
GTATACG803.383973E-416.18751
CTAACGC700.002593301215.8571433
TGCGTAT700.002593301215.85714310
TAATACT7000.015.5928564
CGAACGA957.063427E-515.57894816
CGCATCG1902.7284841E-1115.57894813
TCTATAC2500.015.543
AATAACG1853.0559022E-1015.02
TAACGGC1252.9609437E-614.79999936
CGCTAGA1508.108873E-814.79999937
TCTAACG750.00410567614.7999992
TCGTATT1752.240995E-914.79999926
TTAACGG1252.9609437E-614.79999935
CCGCTAG1651.6467311E-814.57575936
Produced by FastQC (version 0.11.5)