FastQCFastQC Report
Fri 29 Apr 2016
ERR1042519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6111943
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT284090.46481127196376015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT237680.38887797219313075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT190870.312290216057316No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118910.1945535159604728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73200.026.814891
GTATCAA109800.017.8934422
TCTAGCG6450.015.201550528
GACGGAC10850.015.0046087
ACGGACC11050.014.9004528
CTAGCGG6900.014.74637729
TACACCG4150.014.7108435
CTTACAC8850.014.2146893
AAGACGG12550.014.00398355
GCGCAAG13650.013.6886441
ATAACGC6800.013.3308823
CAAGACG13950.013.2616494
CGCAAGA13650.013.146522
TAGCGGC7750.013.12903130
ATCAACG150850.013.0855154
TATCAAC153650.013.015623
CGCAATA7850.012.96178336
TATACTG11750.012.9106395
TCAACGC153350.012.848065
ACGCGCG9850.012.77157421
Produced by FastQC (version 0.11.5)