FastQCFastQC Report
Fri 29 Apr 2016
ERR1042518.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042518.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921055
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT323251.6826691583530922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT274011.4263516661417814No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT198891.035316531801536No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142980.7442785344511219No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70810.3685995455622041No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67000.348766693301337No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64580.3361694485582141No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45220.23539149061323075No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42310.22024356408327714No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28420.1479395436361791No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27470.14299434425354818No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20980.10921082426062763No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG250.005497250729.619
GGTATCA71900.029.5897081
GAGCGTA705.1048664E-621.1428576
ATTCGCG450.003826729320.55555517
CGGTAAG450.003826729320.55555528
TCGCGTC450.003826729320.55555519
GGCGTCG656.905214E-519.9230778
GCGTATC759.271083E-619.7333348
AGAGCGT759.271083E-619.7333345
CGTAGCG2200.019.34090815
GTATCAA111550.019.1053332
CCGGGTA609.2398934E-418.532
GCGCATA701.2197382E-418.526
CCGACCA500.007036559718.59
CGCGTCA500.007036559718.520
CGATCGT609.2398934E-418.526
AACTCGA752.0681768E-417.26666637
TTAACGG1301.3948011E-817.07692335
TCGTAGC2650.016.75471714
TAGCGGA2550.016.68627417
Produced by FastQC (version 0.11.5)