FastQCFastQC Report
Fri 29 Apr 2016
ERR1042517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1681251
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT104070.6190033492916882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT86920.5169959750209815No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62600.37234178596771095No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44100.2623046766961031No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24790.14744972642395454No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21910.13031962508869882No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18600.1106319044568598No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGC250.005497058429.5999983
GGTATCA30800.021.202921
CACGTAC609.2392403E-418.514
GTCGACG852.7243299E-517.41176436
CGGTTTA650.001580345317.07692330
GTACTAG1201.0420081E-716.9583321
TATACCG1107.812887E-716.8181825
TAGACCG700.00259316315.8571425
CTAGCGG1302.5908412E-715.65384629
ATAACGC2201.8189894E-1215.1363643
GTATCAA43600.015.0206422
GTATTAA3700.015.01
TGCGACG750.00410545714.822
GTCGTAA750.00410545714.81
CGGTATA908.276823E-414.38888826
GGATTAG1803.3323886E-914.3888881
TTGCGTG1304.4466196E-614.2307697
ATAGGAC2750.014.1272743
ATTGCGT1051.6563496E-414.0952396
TCTAGCG1458.9162495E-714.03448428
Produced by FastQC (version 0.11.5)