FastQCFastQC Report
Fri 29 Apr 2016
ERR1042516.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042516.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3837874
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT173460.4519689807429843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT140230.36538458531989326No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT112840.29401694792481464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67620.17619129757777352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47050.025.4399591
GTATCAA71750.016.6564462
TATACTG8950.016.3296095
TCTAGCG2650.014.660377528
CCGGTTA908.280233E-414.38888825
GTCGTAC1051.6572243E-414.0952381
CGAACGA2252.7284841E-1113.977777516
TAATACT9200.013.8754
CGTAGAC1753.573041E-813.7428583
TCTATAC5550.013.6666683
ACGCGCG3550.013.54929521
CTAGCGG2900.013.39655129
TAGACAG7400.013.255
CGCGCTA3950.013.11392424
GTACTAG2850.012.9824561
CCGTATA1000.001833838212.9500012
ACGGACC4150.012.9277118
GACGGAC4050.012.7901237
TAGTACC4000.012.48754
TATACAG9050.012.4696135
Produced by FastQC (version 0.11.5)