FastQCFastQC Report
Fri 29 Apr 2016
ERR1042515.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042515.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511883
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81771.5974353514377309No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT66141.292092138242528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48970.9566639251547717No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38420.7505621401765638No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23130.4518610698147819No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21970.429199641324287No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19060.37235071295589034No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.24087535628258802No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12210.23853107057667475No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9160.17894714221804592No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9080.17738428508077042No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.1506203566049273No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA7130.13928964235967986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTCG250.00549353129.632
CGACATT358.8626484E-426.4285728
CAATGCG358.8626484E-426.4285719
ATGCGAC451.3218116E-424.66666821
AGTCCTA400.001929732323.12529
CGACGAC400.001929732323.12525
CACTATC400.001929732323.12536
CCGACGA400.001929732323.12524
TCTATAC705.0928047E-621.1428573
GGTATCA24150.020.9130441
CGTCAGC806.9446105E-720.812515
TCCGACG450.003822952320.55555723
TGCGACG450.003822952320.55555722
GTAAACG450.003822952320.55555727
GTATATA1001.2856617E-820.351
CGGGATA555.13774E-420.18181824
TTACCCT656.892574E-519.9230774
CGACGAG500.007029682418.524
GTCCTAC500.007029682418.530
CCGGTCC500.007029682418.59
Produced by FastQC (version 0.11.5)