FastQCFastQC Report
Fri 29 Apr 2016
ERR1042514.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042514.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068379
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT246932.311258457906791No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT221642.0745447074493226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT160181.4992806859737977No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109761.027350780949457No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55640.5207889709550637No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48650.45536275048461267No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46970.4396379936333455No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33250.31121914601466333No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31710.2968047855676684No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23860.22332898718525918No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22980.21509220978697635No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA18710.1751251194566722No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT15570.1457348001037085No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14920.13965081679815872No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA12900.12074366867937314No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC12300.11512768408963485No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12160.11381728768536259No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11290.10567411003024207No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT10700.10015172518366611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCC250.00549617329.59999824
ATTCGCT400.00193109823.12500210
TTCGCTG502.70193E-422.19999911
GACAATC603.7259702E-521.58333235
CGACGGG555.1427673E-420.18181820
GTGATCG555.1427673E-420.1818188
TCTAGCG555.1427673E-420.18181828
CGTACTC555.1427673E-420.18181833
GACCGTG656.901547E-519.9230777
GTCGTCA951.675835E-719.47368424
ACGAGTC1052.259003E-819.38095326
TGATCGC609.2362356E-418.59
CATTGCG902.1519263E-618.529
CAATGCG1103.8508006E-818.519
CTTAGGC801.616496E-518.53
CTAGCGG609.2362356E-418.529
TATTCGC500.007034566718.4999989
ACCGTGA1002.874458E-718.4999988
CTGTGCG1002.874458E-718.4999989
CGACGAG1351.1514203E-917.81481624
Produced by FastQC (version 0.11.5)