FastQCFastQC Report
Fri 29 Apr 2016
ERR1042513.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042513.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2378415
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT213570.8979509463234969No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT188410.7921662115316292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT120840.508069449612452No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86640.36427620915609765No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34010.1429943891204857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52400.026.4790081
ACGGACC2950.022.5762738
GACGGAC3050.022.4426217
TGCGACG450.00382699320.55555522
CGGACCA3350.019.8805969
GTATACG1254.129106E-1019.241
GTATCAA77950.017.7998712
TACCCCG1054.802605E-717.6190475
CGCAAGA4050.016.4444452
GCGAAAG4100.015.79268418
CGAAAGC4250.015.670588519
GTACTAG1902.7284841E-1115.5789481
TCTATAC3800.015.5789483
TAGCGGA2055.456968E-1215.34146417
CGTAGCG2055.456968E-1215.34146415
GCGCAAG4600.015.2826091
TTGACGG2900.014.67241433
TCGAATT2301.8189894E-1214.47826113
AAGACGG4800.014.2604175
AGACGGA4950.014.2020216
Produced by FastQC (version 0.11.5)