FastQCFastQC Report
Fri 29 Apr 2016
ERR1042512.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042512.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4010571
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT458221.142530577316796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT389200.9704353818944984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT314600.7844269556629218No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197270.49187509708717286No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83020.20700294297245955No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60690.15132508562995145No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58320.1454157026518169No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53260.1327990453229727No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49490.123398887589822No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43900.10946072267515024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128700.024.0773121
TAATACG1502.5283953E-1017.2666664
GTATCAA189850.016.331842
TCTAGCG3350.015.46268728
AATACGC1508.117058E-814.85
CAAGACG7150.014.7482524
TAATACT9450.014.2910054
ACGGACC6300.014.0952388
TATACTG6200.014.0241945
TATTCCG1601.7867569E-713.8755
CTAGCGG3750.013.81333329
CGGTCCA4200.013.65476110
CTTATAC13000.013.51923237
TAACCGG1102.4587518E-413.45454522
AAGACGG6900.013.4057975
CTTACAC6000.013.2583333
TCTATAC4200.013.2142853
CGAACGA4500.013.15555616
GCGACGT2254.0745363E-1013.15555635
GACGGAC6500.013.0923087
Produced by FastQC (version 0.11.5)