FastQCFastQC Report
Fri 29 Apr 2016
ERR1042511.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042511.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4362677
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT292130.6696118002776735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT234620.5377890685008311No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT190500.4366585011908972No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113900.261078232470568No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47790.1095428334483621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77700.025.9047621
GTATCAA118050.017.0660742
CGTAGCG3600.016.44444515
AATACCG1751.3278623E-1015.8571435
CGCGCTA4800.015.80208424
TAGCGGA3900.015.65384717
AATCGGG4450.015.38202223
ACGGACC5450.014.935788
TTAACGG2251.8189894E-1214.835
ACGCGCG5150.014.72815421
GACGGAC5650.014.407087
TCTAGCG3550.014.07042228
CTAGTAC2700.013.7037043
CTAGCGG3650.013.68493229
TAATACT13850.013.6245494
TAACGGC2457.2759576E-1213.59183636
GCGCGCT5600.013.54464323
TACCCCG2900.013.3965515
CTAATAC12500.013.1719993
GCGGAAT4650.013.12903219
Produced by FastQC (version 0.11.5)