FastQCFastQC Report
Fri 29 Apr 2016
ERR1042510.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042510.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4439679
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT390520.8796131432024703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT323630.7289490974460091No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT259260.5839611377309035No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121920.27461444847701827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108950.022.7535551
ACGGACC4750.017.5263168
GACGGAC4900.016.6122447
TAACGGC3400.015.77941236
GTATCAA163750.015.1502282
CGGACCA5500.015.1363639
AATCGTC4550.015.04395628
CGTTCGG1001.0944842E-414.79999924
AAGACGG5600.014.5357145
CGGGTAA3700.014.524
CGTAGCG4800.014.26041615
TTAACGG3900.014.2307735
ACGTTCG2101.364242E-1014.0952387
TAATACT19700.013.8984774
TAACCGG3400.013.602941522
GTATTAG13800.013.5398541
CTAATCG5300.013.26415226
TAGACTA6600.012.8939395
GGACCGT1153.5807615E-412.8695656
CGCAACG1307.006606E-512.80769312
Produced by FastQC (version 0.11.5)