FastQCFastQC Report
Fri 29 Apr 2016
ERR1042509.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042509.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4714291
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT373330.7919112333116475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT320850.6805901460049878No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT239670.5083903390774986No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146570.31090571201480777No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62030.13157864035122144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105900.024.369691
GTATCAA152250.016.8899822
GACGGAC10850.016.7096797
AAGACGG11900.016.478995
ACGGACC11050.016.407248
CGGACCA11450.015.3493449
TCTAGCG5200.015.29807728
CGCAAGA12050.015.0456422
CAAGACG13400.014.9104484
GCGCAAG13200.014.5757591
TATACTG8150.014.0736195
CTAGCGG5400.014.04629729
AGACGGA13350.013.8576786
TACACCG3100.013.7258075
CTAGGAC5400.013.3611123
TAGACTG4450.013.3033715
GTATTAG11150.013.1076231
ACGCGCG10200.013.05882421
CGCAATA6450.012.90697736
GCAAGAC18550.012.7654983
Produced by FastQC (version 0.11.5)