FastQCFastQC Report
Fri 29 Apr 2016
ERR1042508.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042508.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5262734
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT543261.0322771396008235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT462260.8783647434964412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT375020.7125953924329066No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239540.45516265880053985No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100210.19041433597061908No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71480.13582293917952154No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69910.13283969890935016No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65830.12508707451298126No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64880.12328192912657186No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54160.10291228855572028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172850.020.1000861
ACGGACC6550.017.7938948
GACGGAC6800.017.4117667
GTCGATA1151.2441869E-616.08695625
CGTATAC1751.3278623E-1015.8571433
TACGACG7000.015.8571435
CGGACCA7850.015.3184719
TAAGTCG750.004107001714.85
CTAGCGG4900.014.34693829
GTATCAA244600.014.211572
GACGGTA7900.014.0506328
CGTAGCG3000.013.56666715
TAACGCC10100.013.5544564
TAGGTCG1102.458981E-413.45454521
GTATTAG11650.013.3390551
CGCAAGA9600.013.2968742
ATGGTCG8350.013.29341336
TCTAGCG5050.013.18811928
AAGACGG9300.013.1290325
AGTACCG3550.013.0281695
Produced by FastQC (version 0.11.5)