FastQCFastQC Report
Fri 29 Apr 2016
ERR1042507.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042507.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13076219
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT638350.4881762839854548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT440080.336549884947629No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT429150.3281911996120591No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200390.15324766279916235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA190150.023.3986321
TTAACGG10450.016.46411535
ACGGACC22950.016.3638348
TCTAGCG17900.016.01955428
GACGGAC23350.015.8458257
GTATCAA281500.015.7726462
TAACGGC11100.015.66666736
CTAGCGG18600.015.51612929
AAGACGG28350.014.94356255
GTATTAG49550.014.0756821
AGACGGA27500.014.0599996
CGGACCA26550.014.00565059
CGCAAGA27950.013.7012522
TACCGTC16900.013.2455637
TCTATAC16200.013.1327163
TTTAGCG9050.013.08287226
TAATACT49400.012.957494
TAACCGG9450.012.92063522
CGCAATA24050.012.69230736
AACGCAG354350.012.6030767
Produced by FastQC (version 0.11.5)