FastQCFastQC Report
Fri 29 Apr 2016
ERR1042506.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042506.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9202719
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT754100.8194317353382191No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT550600.5983014367818902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT434430.4720670054143781No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234000.25427267745543464No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC108830.11825852772425194No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG100770.10950024661189807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA291050.018.5031781
ACGGACC13300.015.7180448
GACGGAC13550.015.4280447
CGTAGCG7000.015.32857115
TTTAGCG6950.014.64028726
TATACTG14050.014.6156595
CGGACCA14350.014.5679459
ATTGACG18200.014.43406732
ATAACGA11950.014.24267812
AAGACGG16150.014.2043345
CGAACGA11500.014.15652216
CTAGCGG5900.014.1101729
GCGGAAT8100.013.93209819
GTATCAA388050.013.83507252
TTGACGG19250.013.64675233
AGTACCG11850.013.5822795
CGCAAGA16100.013.5590072
AATCGGG8750.013.53142823
TAACGAA12350.013.48178213
CGATAAC12400.013.4274210
Produced by FastQC (version 0.11.5)