FastQCFastQC Report
Fri 29 Apr 2016
ERR1042504.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042504.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15420761
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT451380.292709289768514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT319340.20708446230377348No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT313460.20327142091106915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152700.022.716111
GACGGAC28750.017.6956527
ACGGACC30550.017.0163678
TCTAGCG20750.016.67228928
CGGACCA32750.015.8167959
CTAGCGG22050.015.689342529
GTATCAA224600.015.4441232
CGCAAGA34550.015.3675822
AAGACGG38300.015.0221935
GTATTAG48100.014.6153851
CGCAATA24650.014.33468636
AGACGGA37450.014.2763686
CGAGCCG33800.014.17603615
TTAACGG10050.014.17412935
TATACTG26250.013.9542855
GCGCAAG40500.013.5666671
TAACGGC10850.013.47004636
CAAGACG45300.013.2726274
TCTATAC18700.013.1577543
TAATACT45300.013.0684334
Produced by FastQC (version 0.11.5)