FastQCFastQC Report
Fri 29 Apr 2016
ERR1042503.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042503.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3732450
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT360880.9668716258757654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT242790.6504842663665956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT154140.4129727122935337No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95230.25514072526088766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91300.025.632531
GTATCAA122100.019.1212122
GCCGAGT4850.019.07216512
CGGAACC4000.017.57514
ACGGACC6600.017.3787888
GCTCGGA10050.017.11940411
CCGAGTT5550.016.66666613
CCGGAAC4350.016.58620613
CCGGGTA700.002593957415.85714332
CGTATCC700.002593957415.8571433
CGTCGTA4100.015.79268210
GACGGAC6850.015.6642337
CGCAAGA7500.015.5399992
TATATCG2750.015.4727275
CGTATCG3000.015.41666710
CGGACCA7100.015.37323959
CCGTCGT4250.015.2352949
CGAGTTC6600.015.13636514
CTCGGAG12000.015.10833412
GCTCCGA11650.015.08583629
Produced by FastQC (version 0.11.5)