FastQCFastQC Report
Fri 29 Apr 2016
ERR1042502.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042502.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8490295
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT405520.4776276913817482No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT285020.3357009385421826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT284390.3349589148551375No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121930.14361102882762022No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC101650.1197249329970278No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG96220.11332939550392537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC12600.018.0595257
ACGGACC13050.017.5785438
GGTATCA192850.017.2193411
AAGACGG14400.016.701395
TCTAGCG11450.016.4803528
CTAGCGG11550.016.33766229
CGTAGCG5750.015.76521815
TATACTG12100.015.7479335
CGGACCA14700.015.4795919
TAGCGGA5800.015.31034517
GTATTAG26050.014.9846461
TCGTAGC6700.014.91044714
CGGTCCA13300.014.60526310
TTAACGG6000.014.49166635
TAACGGC6300.014.09523936
GTATAGG19400.013.6365981
TCTATAC11200.013.5446433
TAGTACG5250.013.3904754
CGCAATA13850.013.35740136
AGACGGA17350.013.3285296
Produced by FastQC (version 0.11.5)