FastQCFastQC Report
Thu 28 Apr 2016
ERR1042501.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042501.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7704696
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT250270.32482787120997375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT169890.2205018861224375No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT107020.13890230062289285No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT104410.13551475619544237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89150.023.137971
ACGGACC24450.019.7484668
GACGGAC24250.019.6061867
CGGACCA25250.019.0495059
AAGACGG27950.018.4669065
GTATCAA113750.018.1178042
CGCAAGA28050.017.675582
TATACTG12950.016.9999985
GCGCAAG29100.016.8470781
AGACGGA29300.016.6058036
TACGACG11300.015.7168145
TCGTTTA13100.015.5343530
ATGGTCG12550.015.47808836
GCGAAAG30550.015.38134218
CAAGACG34600.015.3453754
ATAACGC15200.015.0921063
CAGAGCG31900.015.02037514
CGACGGT11450.014.864637
ACGACGG11600.014.8318986
AGCGAAA32500.014.68615317
Produced by FastQC (version 0.11.5)