FastQCFastQC Report
Thu 28 Apr 2016
ERR1042500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19513551
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT475460.24365631862698903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT329060.16863153200563033No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT313150.16047822356884198No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT204330.10471184870452332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159350.024.3919051
ACGGACC48600.017.4341568
GACGGAC48500.017.2412367
AAGACGG54500.016.6330265
TTAACGG16200.016.2160535
GTATCAA240450.016.0956542
TCGTTTA39450.015.85044430
TAACGGC16600.015.82530136
CGCAAGA54500.015.8183482
CGGACCA53850.015.73444759
AGACGGA57450.015.2315056
TAACGCC44950.014.4872074
CAAGACG66950.014.3689324
CGAGCCG48700.014.13141715
CGTTTAT44400.014.04166731
CGCATCG44200.014.02149413
ATAACGC49150.014.0020343
TACGACG34700.013.9683015
ATGGTCG41800.013.94138736
CGAACGA25700.013.89299616
Produced by FastQC (version 0.11.5)