FastQCFastQC Report
Thu 28 Apr 2016
ERR1042499.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042499.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8562731
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT712650.8322695177508204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT611590.7142464244176303No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT486500.5681598546071341No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283780.3314129569176002No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT103010.12030040415843965No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC93760.10949777588481993No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87030.1016381339084458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG25300.015.8675895
CAAGACG28150.015.1154534
TCTAGCG14050.014.87900428
ACGGACC25650.014.7855758
CGAACGT7500.014.5533324
GACGGAC26300.014.4904957
CGCAAGA27400.014.4489052
CTAGCGG14750.014.298305529
TCGAACG7800.014.230773
CTAGTAC5600.014.2053583
CGAGCCG22400.014.04017915
GCGCAAG28750.013.899131
CGGACCA27600.013.8759
GGTATCA385000.013.5122081
TACGACG19300.013.4196895
CGGTCCA16700.013.18263410
GCGTTAT19350.013.098191
GCGGGTA11050.013.05882423
CAAATCG20950.012.980906513
TAACGCC13000.012.8076934
Produced by FastQC (version 0.11.5)