FastQCFastQC Report
Thu 28 Apr 2016
ERR1042498.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042498.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20911994
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1667720.797494490482352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1572460.7519416847575607No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1250540.5980013192429187No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT728130.348187743359146No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248600.11887914657970923No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT240480.11499620743961575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG59500.017.3806725
CAAGACG66250.016.335854
GACGGAC61100.016.3199677
TCTAGCG35050.016.25677728
ACGGACC62950.015.72279558
CTAGCGG36650.015.59754529
CGGACCA65150.015.2770539
CGCAAGA67550.015.227242
GCGCAAG70000.014.8264281
GTAAACG42600.014.63497627
CGGTCCA41750.014.35688610
AGACGGA72050.014.2505216
TTCGGGC43650.014.24055135
CGCAATA41350.014.13784736
TAAACGC44600.014.02017928
CGCTTCG45950.013.9706232
CGAGCCG51050.013.5533815
ACGCTTC47550.013.53943331
CGAACGA36800.013.37228316
TAGCGGC43400.013.34216630
Produced by FastQC (version 0.11.5)