FastQCFastQC Report
Thu 28 Apr 2016
ERR1042497.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042497.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10553423
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT495340.46936430009486024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT385760.3655306908478889No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT334840.3172809428751221No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157960.14967655518024814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA185550.020.3594721
AAGACGG22950.017.4117645
ACGGACC21550.016.9976798
GACGGAC21400.016.684587
TCTAGCG14800.016.37528
CTAGCGG15200.016.187529
CGGACCA23450.015.699369
AGACGGA26700.014.619856
GTATTAG30350.014.4464581
CGGTCCA18550.014.36118610
GTATCAA263350.014.2956142
CGCAAGA26950.013.9350652
CAAGACG29800.013.906044
CGCAATA18900.013.80158736
GTAAACG16450.013.72036627
TATACTG18250.013.4821915
TAAACGC17300.013.36705328
CGAACGA14450.013.31487816
GCGTTAT19800.013.2676771
GCGCAAG29200.013.1780821
Produced by FastQC (version 0.11.5)