FastQCFastQC Report
Thu 28 Apr 2016
ERR1042496.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042496.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3952017
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT306780.7762618430032058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT264560.6694303187460986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT204180.5166475751496008No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104570.2645990642246731No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT47220.11948329169636669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG11650.018.1030045
CAAGACG13100.016.9465664
GACGGAC12350.016.7773277
TCTAGCG7650.016.68627428
ACGGACC12500.016.5768
CGCAAGA12750.016.5411762
CTAGCGG7750.016.47096629
GCGCAAG14100.015.744681
CGGACCA13550.015.2915139
AGACGGA13850.015.0938626
CGGTCCA9050.014.92265110
CGCAATA8850.014.84180736
GTATACG1904.5656634E-1014.6052641
CGCTTCG10150.014.58128132
TAAACGC9700.014.30412428
GCAAGAC19400.014.2087633
TTCGGGC10200.014.14705935
CCGGTCC10050.013.990059
GTAAACG9800.013.96938927
TAGCGGC9300.013.92473130
Produced by FastQC (version 0.11.5)