FastQCFastQC Report
Thu 28 Apr 2016
ERR1042495.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042495.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4680897
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT463220.9895966520946733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT427890.914119665525646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT330450.7059544356562428No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174150.37204407616745255No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54850.11717839550838226No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA48750.10414670521483382No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT47410.10128400603559531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC10100.016.8514848
CTAACGC904.450653E-516.4444453
AAGACGG10950.016.050235
TGCGACG1059.355292E-615.85714222
TAACGGC2500.015.54000136
GACGGAC10550.015.4312797
TTAACGG2400.015.41666735
CGGACCA11050.015.2352949
TCTAGCG6800.014.96323628
CGTTATT7300.014.9520552
CTAGCGG6850.014.85401429
CGAGCCG10750.014.815
AGACGGA11700.014.7051296
GCGTTAT8450.014.6686381
TTAGACT4300.014.6279074
CGCAAGA11300.014.5707972
TCGAACG3650.013.6849323
GGTATCA229450.013.6663761
CGGTCCA7600.013.63157910
TAAACGC9950.013.57286528
Produced by FastQC (version 0.11.5)