FastQCFastQC Report
Thu 28 Apr 2016
ERR1042494.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042494.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5273582
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT313820.595079397646609No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT261050.4950145840151912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT230950.4379376294897851No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106450.20185520960895267No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA53720.1018662457509905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113700.019.8342131
TCTAGCG5050.017.95049528
CTAGCGG5300.017.45283129
GTATTAG12950.015.9999991
TAACGGC3050.015.77049336
ACGGACC8900.015.5898898
GTAAACG6850.015.39416127
TTAACGG3250.015.36923135
TAATACT14250.015.1894744
CGCTTCG7600.014.84868432
AAGACGG10300.014.3689335
TTAGGAC6850.014.3138693
TAAACGC7500.014.30666728
GACGGAC9150.014.1530057
CGGACCA9500.014.0210529
CGTGCGA3300.014.01515110
GTATCAA164300.013.6920262
TAGTCCG3000.013.5666665
TAGGACA5900.013.483054
ATAATAC11350.013.2026433
Produced by FastQC (version 0.11.5)