FastQCFastQC Report
Thu 28 Apr 2016
ERR1042493.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042493.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099476
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT241541.1504775477309575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT219261.0443558297403734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT174830.8327315958839253No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95870.45663775151513997No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC43500.20719455711806184No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA36150.17218582160501No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31010.14770352221220914No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27780.13231873095953467No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG27420.1306040173833852No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA27290.12998481525866454No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26680.1270793283657446No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG22750.10836037182611279No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21000.10002495860871952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTG650.001580521817.0769235
TAACGGC1107.8151606E-716.81818236
TTAACGG1107.8151606E-716.81818235
TACACCG1352.2220775E-816.4444455
TCGAACG1708.54925E-1116.3235283
TCTAGCG3250.015.93846128
CGAACGT1954.1836756E-1115.1794874
CGATGCG4450.014.96629133
CGTATCG750.00410590914.810
ATAGGGG2006.184564E-1114.83
AAGACGG4250.014.85
GTATTAG5600.014.5357151
CTAGCGG3700.014.529
TAGTACG908.27803E-414.3888894
CAAGACG4400.014.2954544
CGAACGA3150.014.09523916
TACCCCG1051.6566602E-414.0952385
ACACCGT1051.6566602E-414.0952386
CGAACTA1458.919287E-714.03448329
TAACGAA3200.013.87513
Produced by FastQC (version 0.11.5)