FastQCFastQC Report
Thu 28 Apr 2016
ERR1042492.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042492.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4160167
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT421751.0137814179094253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT364480.876118675043574No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT317960.7642962409922486No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157310.37813385856865844No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47960.11528383355764323No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA47940.11523575856449994No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45390.10910619693872865No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC43620.10485156004554626No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT42630.10247184788495269No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42190.1014141980358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG11600.018.1810345
TCTAGCG6950.017.56834628
CTAGCGG7600.016.30921229
CGCAAGA13350.015.9363292
ACGGACC12200.015.9221328
CGGTCCA8650.015.61271810
GACGGAC12550.015.4780877
GTACGAC855.368348E-415.2352933
GCGTTAT7200.015.1597221
CGGACCA12700.015.1496079
GCGCAAG14350.014.8257841
CGCAATA8750.014.836
TTCGGGC7500.014.55333235
CAAGACG14150.014.5123684
CGTACGG908.280442E-414.38888822
TAGCGGC8900.013.92696630
AGACGGA14500.013.779316
TATACTG8600.013.7674425
AGCGGCG9750.013.66153831
CCGGTCC9750.013.6615389
Produced by FastQC (version 0.11.5)