FastQCFastQC Report
Thu 28 Apr 2016
ERR1042490.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042490.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8643822
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT427870.49500093824236546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT372900.4314063848144953No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT311730.36063907840767656No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169690.19631362145125153No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT97440.11272791133366698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG23000.017.7760875
ACGGACC23300.016.4356238
TCTAGCG15200.016.43092228
CTAGCGG15450.016.2847929
GACGGAC23500.016.0595747
CAAGACG26150.015.776294
CGCAAGA25600.015.4648442
TAAACGC23050.015.16919828
AGACGGA25800.015.1298466
TTCGGGC22850.014.97811935
GTAAACG23550.014.76857827
CGGACCA26450.014.6181479
CGAACGA12000.014.49166616
ACGCTTC24800.014.47177331
CGCTTCG25300.014.40513832
GCGCAAG28700.013.9878041
CTTCGGG25300.013.96640234
GCTTCGG25850.013.81237933
TACGACG18550.013.7628035
ATAACGA13000.013.66153812
Produced by FastQC (version 0.11.5)