FastQCFastQC Report
Thu 28 Apr 2016
ERR1042489.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042489.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12057389
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT630820.5231812625436568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT474900.3938663669223909No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT415290.34442780273573326No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232020.19242972089562674No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT186360.15456082573101024No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT145830.12094658304546697No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA125640.10420166422431922No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC121830.10104177612582624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG41900.018.146785
CAAGACG45600.016.9177654
GACGGAC44400.016.5833347
ACGGACC44750.016.4949728
ACGAACG19200.016.187515
CGAACGA18800.016.13829816
CGCAAGA47300.016.0750542
TCTAGCG25400.015.65944928
CGGACCA48400.015.4039259
AGACGGA48850.015.3377696
GCGCAAG51900.015.3275531
TTAACGG4400.014.29545435
CTAGCGG27850.014.28186729
TAAACGC31800.014.25314528
AACGAAC21700.014.23732814
TAACGAA21500.014.19767413
TTCGGGC32900.013.94528835
ATAACGA21900.013.93835712
GTAAACG32550.013.81106127
GCAAGAC59150.013.7928983
Produced by FastQC (version 0.11.5)