FastQCFastQC Report
Thu 28 Apr 2016
ERR1042488.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042488.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16852617
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT727730.4318201736857842No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT579350.343774501016667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT497890.2954377946167055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283850.16843081403914892No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT185710.11019653505446661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG48600.016.406385
TCTAGCG30000.015.72499928
CAAGACG53000.015.3235854
GACGGAC47650.015.1416597
CGCAAGA50950.014.9960752
ACGGACC48400.014.9834728
CTAGCGG31750.014.74173429
CGGACCA49450.014.6653199
GGTATCA381250.014.5041
AGACGGA53450.014.3638926
CGGTCCA34000.013.87510
CGAACGA23050.013.72451216
TATACTG29150.013.644945
GTAAACG41450.013.61278627
TAAACGC41600.013.51923128
ACGAACG23550.013.43312115
ACACGCT43450.013.4119689
TTCGGGC42650.013.40328235
CGCAATA35200.013.40198936
GTATTAG52350.013.3581661
Produced by FastQC (version 0.11.5)