FastQCFastQC Report
Thu 28 Apr 2016
ERR1042487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11513848
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT595060.5168211357315122No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT508580.4417115806983034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT410080.35616242284942445No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227340.19744919335395084No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT139950.12154928569493013No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA127380.11063199722629653No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC123350.1071318641691292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG31300.017.9089455
GACGGAC31850.016.4379927
CAAGACG35800.015.9678764
CGCAAGA35700.015.6498592
TCTAGCG18350.015.62670328
AGACGGA35050.015.5706146
ACGGACC33750.015.5125918
CGGACCA34600.015.2919089
CTAGCGG19650.014.87531829
TTCGGGC25000.014.4335
CGAACGA20150.014.3225816
GGTATCA296500.014.3195611
GTAAACG25900.013.92857227
TAAACGC25700.013.89299728
TTAACGG5500.013.45454635
GCGTTAT25800.013.2655041
ATAACGA22750.013.17362712
CGTTATT24300.013.1707822
ACGCTTC28350.013.11640231
ACGAACG22500.012.99111215
Produced by FastQC (version 0.11.5)