FastQCFastQC Report
Thu 28 Apr 2016
ERR1042486.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042486.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12542833
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT513140.40911012687484555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT445610.35527061549810957No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT360090.2870882519124667No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208580.16629416974618094No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT180520.14392282827970362No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC141220.11259019393784482No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT135820.10828494647102452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG42600.018.4565735
CAAGACG44550.018.0224464
GACGGAC43400.017.1359447
ACGGACC44700.016.7203588
CGCAAGA46350.016.5641862
TCTAGCG24600.016.168728
CGGACCA46600.015.8004289
AGACGGA49450.015.6380186
GCGCAAG49800.015.4538151
CTAGCGG26050.015.19769729
GCAAGAC61250.014.6791843
GTAAACG39550.014.54740927
TAAACGC39600.014.48232428
CGAACGA18050.014.45152416
TATACTG22850.014.2494535
TTAACGG4450.014.1348335
ACGAACG18450.014.03794115
CGAGCCG45750.013.82950915
GGTATCA269550.013.7952151
TTCGGGC41250.013.6339435
Produced by FastQC (version 0.11.5)