FastQCFastQC Report
Thu 28 Apr 2016
ERR1042485.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042485.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9839442
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT697890.7092780261319697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT684470.6956390413196196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT505470.5137181559686007No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291980.29674446985916475No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT123990.12601324343392645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG30300.019.0495055
CAAGACG32850.017.514464
GACGGAC30750.017.2065037
ACGGACC30850.016.9108588
CGGACCA32600.016.1165649
AGACGGA34400.016.0261636
CGCAAGA33700.015.9198822
TCTAGCG18200.015.85714328
CGAACGA12400.015.516129516
ACGAACG12800.014.88671915
GCGCAAG36050.014.83079051
CTAGCGG19600.014.72448929
ATAACGA13900.014.10791412
TAAACGC24600.013.98780428
TTCGGGC24700.013.93117335
ACCGTCG19400.013.827328
TAACGAA14450.013.8269913
GTAAACG24650.013.8093327
TACCGTC19800.013.7348497
GCAAGAC45800.013.6124453
Produced by FastQC (version 0.11.5)