FastQCFastQC Report
Thu 28 Apr 2016
ERR1042484.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042484.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8355764
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT572950.6856943302850583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT557490.6671921322813809No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT426090.5099354170366708No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243800.29177463604764325No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT137940.1650836476473007No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC98720.11814598880485375No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT95560.1143641682555898No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA87280.10445484099359437No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC83850.10034989020752622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG31850.017.657774
AAGACGG32600.017.5920245
GACGGAC31650.017.1263837
ACGGACC31950.017.0813798
TCTAGCG19750.016.7670928
CTAGCGG19900.016.54773929
CGGACCA34150.016.1976579
CGCAAGA35450.016.1777152
GTAAACG26600.015.71804527
AGACGGA35400.015.6779666
TAAACGC27350.015.62522828
TTCGGGC26350.015.4459235
CGAGCCG31200.015.41666715
GCGCAAG38150.015.1782441
GCTTCGG27550.015.17604333
CGCTTCG28250.014.93097332
CGGTCCA22900.014.70305710
ACGCTTC29200.014.50856131
CTTCGGG29800.014.21644334
AAACGCT30500.014.19344229
Produced by FastQC (version 0.11.5)