FastQCFastQC Report
Thu 28 Apr 2016
ERR1042483.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042483.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6842911
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT369520.5400041005940308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT350710.5125157991971546No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT232330.3395192484601948No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157810.23061822665821607No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT145330.21238037437575907No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC90610.13241440667575538No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG88370.12914094600967335No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA85290.1246399375938106No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC85110.124376891647429No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC80700.11793226596108003No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT80610.11780074298788923No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA79520.1162078536459118No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG79010.11546255679783064No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA76640.11199911850380635No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT75860.1108592527361528No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG73130.10686972254936532No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG73050.10675281323986238No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC71250.10412235377604648No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG71090.10388853515704063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG32050.019.6833085
CAAGACG31350.019.6507194
GACGGAC34400.017.5319777
ACGGACC34800.017.2772988
CGCAAGA36400.016.8736272
CGGACCA36050.016.7295429
AGACGGA37850.016.5204756
GCGCAAG36850.016.516961
TAGAGTG18050.015.9889195
GCAAGAC41900.015.8508363
GGTTATC12750.015.8156862
CGAACGA13200.015.55681816
TTAGAGT17050.014.9736074
TCTAGCG15150.014.77557828
TACCGTC22000.014.715917
CGCTTCG22200.014.66666732
ACCGTCG21700.014.6635948
AACGAAC14050.014.61565914
ACGAACG14050.014.61565915
CGTCGTA21850.014.39359310
Produced by FastQC (version 0.11.5)