FastQCFastQC Report
Thu 28 Apr 2016
ERR1042482.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042482.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19439900
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT612310.31497590008179055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT483930.24893646572256029No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT457140.23515553063544564No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257360.13238751228144177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172500.026.9295641
GTATCAA275700.016.8023222
TCTAGCG22450.015.57461128
GACGGAC34100.015.51612957
CTAGCGG23050.015.16919829
ACGGACC36150.014.840948
CGAACGA14100.014.30141716
AAGACGG43800.013.6004565
TACCGTC23300.013.3390557
CGGACCA40800.013.3308829
TAATACT41450.013.3003624
CGCAATA27700.013.22382636
TAAACGC28550.013.08931728
ATAACGC24350.013.0677623
GTAAACG29000.012.88620727
TATACTG40650.012.8794585
GTATTAG44200.012.7658371
TCTATAC25300.012.723323
ACCGTCG22550.012.7161878
CAAGACG48550.012.5746654
Produced by FastQC (version 0.11.5)