FastQCFastQC Report
Thu 28 Apr 2016
ERR1042481.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042481.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11861635
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT471760.397719201442297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT373110.31455191463908644No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT252770.21309878444244826No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194640.16409204970478353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136950.023.4779111
GTATCAA186650.017.1768022
ACGGACC17300.014.5433528
GACGGAC17750.014.3830987
TATACTG24350.013.5236145
TACCGTC10250.012.9951237
ATCAACG252200.012.9617374
AAGACGG23050.012.9219095
TCAACGC253200.012.8740125
TAGACAG21100.012.6255935
CAACGCA261000.012.4750966
TATCAAC265150.012.3705443
ACCGTCG9350.012.2673798
AACGCAG268300.012.1287737
CGAACGA6950.011.97841716
TAATACT20800.011.9182694
CGGACCA21200.011.8679259
ATACCGT15300.011.8496736
TACACCG12900.011.759695
CAAGACG26550.011.6365354
Produced by FastQC (version 0.11.5)