FastQCFastQC Report
Thu 28 Apr 2016
ERR1042480.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042480.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19042818
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT767230.402897302279526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT600110.3151371819023844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT560620.294399704917623No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297440.15619536982394097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA334800.016.7649351
GACGGAC37800.016.2486787
ACGGACC37850.016.2272138
TTAACGG11000.015.64090835
AAGACGG43350.015.1499425
TCTAGCG24450.014.98159428
CTAGCGG24300.014.92181129
GTAAACG31850.014.34693827
TATACTG33400.014.1796415
CGGACCA44400.014.0416679
TAATACT46250.014.044
TAAACGC32950.014.03641928
AGACGGA46800.013.5982916
CGCAAGA48050.013.4755462
CAAGACG52700.013.3396594
TAACGGC13200.013.31439336
GTATTAG54650.012.7282711
TACGACG27400.012.4908755
CGACGGT26750.012.3794397
CGAGCCG44050.012.34733215
Produced by FastQC (version 0.11.5)