FastQCFastQC Report
Thu 28 Apr 2016
ERR1042479.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042479.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7284635
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT567320.7787898775985345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT428330.5879910249449698No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT370670.508838122980767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192570.2643509249262317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136800.026.9791661
GTATCAA208400.017.68332
ACGGACC8300.014.7108438
GACGGAC8450.014.230777
TTAGACT7850.014.1401274
TATACTG14500.014.0344835
CGAACGA7150.013.71328616
ATCAACG278700.013.3024774
CGTATAC2408.185452E-1113.1041673
TCAACGC283500.013.0772495
TCTATAC8450.012.917163
TATCAAC288150.012.8854763
CAACGCA288250.012.8553346
AAGACGG11200.012.7187515
CGCGCTA10400.012.45192324
TAGGACG3300.012.3333334
AACGCAG300200.012.3251167
ACGCGCG10450.012.21531121
GCGCGCT11050.012.05429823
GTTGCGC14900.012.04362432
Produced by FastQC (version 0.11.5)