FastQCFastQC Report
Thu 28 Apr 2016
ERR1042478.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042478.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3558892
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT198680.558263639357418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT143480.4031591854993071No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130530.3667714558351307No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59580.16741165508815664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34600.030.0491311
GTATCAA57400.017.8231722
TAATCCG1254.8499627E-513.325
ATCAACG77500.013.2483874
TATCAAC79250.013.1659313
TCAACGC78300.013.1366545
CGACTAA1000.00183379312.94999937
TATACTG8750.012.8971435
TAGGTCG1153.5804504E-412.86956521
CAACGCA80450.012.8085776
CTAGTAC3100.012.5322583
GTACTAG2704.3655746E-1112.3333341
AACGCAG84350.012.2602257
GCTATAC3400.011.9705883
GTTCGTA1757.344146E-611.628571525
TAATACT9100.011.5879124
TATTCCG1150.005222114711.260875
CGTACGA1150.005222114711.2608710
GTGTAGA8250.011.2121221
CTATACT7950.011.1698124
Produced by FastQC (version 0.11.5)