FastQCFastQC Report
Thu 28 Apr 2016
ERR1042477.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042477.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12914733
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT291460.22568023667233383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT242880.1880642828620615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT231090.1789351742695726No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129900.10058279950503042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC11200.014.37053536
TTAACGG10850.014.3225835
AAGACGG33650.014.1842495
TCGAACG12500.014.063
GTATTAG47750.014.0251311
TCTAGCG21050.013.88598628
CGAACGT12450.013.6706834
CGCGTAT950.00124628213.63157914
GACGGAC31650.013.5608227
CAAGACG38050.013.2247044
ACGGACC33350.013.2023988
CTAGCGG22500.013.07333429
CGCAAGA35900.013.0376042
TAATACT34700.012.9020184
ATTAGAG37100.012.8652293
TATACTG25850.012.6673125
TTAGACT13600.012.5147064
ATAACGC23700.012.4894513
GCGCAAG38950.012.44415951
GTGTAAG21700.012.3617521
Produced by FastQC (version 0.11.5)