FastQCFastQC Report
Thu 28 Apr 2016
ERR1042476.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042476.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3628857
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT358810.9887686398224014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT279200.7693882674351732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT265640.7320211295181926No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153670.4234666728394092No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC94100.259310300736568No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA57300.157900958896975No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG55000.15156287503200042No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51200.14109125821160767No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG49110.13533186896039165No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA45890.12645855154942726No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45300.12483269525362946No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC40310.11108180895527159No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40100.11050311434151305No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA39730.10948350954584321No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38890.10716873109080903No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT37570.10353122209004102No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG36920.10174002447602647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG7700.023.5454542
TAACGCA8500.021.5470564
GTATAAC9250.021.01
ATAACGC10900.020.1972473
TTAACGG2200.017.6590935
CGTACGG1455.3556505E-815.31034522
TAACGGC2600.014.94230736
TATACTG4350.014.88505655
TATGTCG750.00410667614.7999995
CGTGACT1152.2123317E-514.47826112
CACGCAG6650.014.4661655
ATCACGC6650.014.4661653
TCACGTA2750.014.12727225
ATAGGGG5900.014.1101693
TATTAGA5900.014.1101692
AGTACCG3950.014.0506325
TAGGGGT6750.013.97777754
TCACGCA7000.013.7428584
TTAGCGA1356.5758522E-613.70370327
GTAATAC4350.013.6091953
Produced by FastQC (version 0.11.5)