FastQCFastQC Report
Thu 28 Apr 2016
ERR1042475.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042475.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6224711
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC528840.8495816110980896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT385810.6198038752321192No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG344620.5536321284634741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT341060.5479129874463249No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA337710.542531211489176No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT295920.4753955645491011No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG208220.3345054894918013No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG196620.31587008617749485No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC187090.3005601384546206No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184760.29681699278890217No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT165530.265923992294582No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG157170.25249364990599565No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA144660.23239633133168752No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC125900.20225838597165394No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT98870.15883468324874842No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG87640.14079368504015688No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT85670.1376288794772962No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC83350.13390179881443492No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC82420.13240775354871898No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72400.11631062068584389No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT69400.11149111982869567No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC67030.10768371415154857No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA66560.106928659017262No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG65230.10479201363725962No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC64680.10390843848011577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG8350.020.8263472
TATCACG8400.018.279762
GTATAAC10750.017.8976751
TAACGGC7550.017.88741936
TTAACGG7550.017.64238535
AAGACGG16000.016.7656235
TAACGCA10750.016.6930244
AATCGTC2450.016.61224628
TCACGCA9950.016.1758794
TTAGCGA2300.016.08695627
ATTAGAG18100.015.43370153
ACAACGC4900.015.1020413
ATCACGC10550.015.0805683
CGCAAGA16600.014.9337342
ATAACGC19700.014.8375633
GTATCAC11000.014.81
GTATTAG22450.014.5857471
CGTTATA1651.6492777E-814.5757572
TCACGTA8600.014.4127925
TATACTG6550.014.404585
Produced by FastQC (version 0.11.5)