FastQCFastQC Report
Thu 28 Apr 2016
ERR1042474.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042474.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19372629
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT631960.3262128232569777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT532130.2746813558448882No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT497970.2570482302634299No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC457780.23630246571077163No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG331740.17124160071407965No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA286410.14784260824898882No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263320.1359237303310769No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG199460.10295969638400652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG11250.017.9244442
TAACGGC24900.017.4598436
TTAACGG25200.017.17857235
AAGACGG47700.016.6771495
TAACGCA14300.015.9125874
GACGGAC49050.015.5015297
ACGGACC49800.015.30522258
CGGACCA52450.014.9199249
CGCAAGA53100.014.7372882
GTATTAG76900.014.6267891
ATTAGAG62550.014.25579553
TCTAGCG32400.014.10339528
GCGCAAG58200.013.8908941
TAATACT61300.013.85236554
CAAGACG65050.013.7932364
AGACGGA56700.013.7363326
GGGGTTA45550.013.64654356
ATAGGGG42800.013.3563083
ATAACGC43150.013.333723
CGAACGA24600.013.31097416
Produced by FastQC (version 0.11.5)