FastQCFastQC Report
Thu 28 Apr 2016
ERR1042473.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042473.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4408808
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT269910.6122062924944792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT219800.4985474531891613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT209710.4756614486273841No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114670.26009297751228905No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC85900.19483724398975868No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG55210.12522659185884255No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA54760.12420590781000217No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT48290.10953073937445222No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46040.10442731913025016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAT1052.2637323E-819.38095316
TATAACG2850.017.5263162
TCTAGCG9050.016.3535928
AAGACGG11700.016.2863255
GTATTAG18150.016.0027541
TAACGGC5550.015.66666836
TTATGCG5100.015.5980414
TATGCGG5250.015.5047625
TACACCG2400.015.4166675
TTAACGG5750.015.12173935
CTAGCGG9600.015.03125129
ATAACGC9650.014.9533683
TATTAGA9750.014.82
TCACGTA5500.014.825
GACGGAC12700.014.7125997
GTACCGT908.280576E-414.3888896
ATTAGAG12750.014.3647073
CGCAAGA13200.014.2954542
ATAGGGG9100.014.02747253
TATCACG4750.014.0210522
Produced by FastQC (version 0.11.5)