FastQCFastQC Report
Thu 28 Apr 2016
ERR1042472.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042472.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3626497
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT245480.676906667784366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT206130.5683997532605156No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT140600.3877019614244821No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100880.2781747785810936No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC87890.24235508811947173No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA63430.17490707975216854No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG60280.16622101162637112No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG44570.12290097027517188No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG43690.12047438616383799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA3500.018.516
AAGACGG9050.016.558015
ACGAACG3850.016.33766215
TAACGCA3850.015.8571424
TATAACG3850.015.8571422
GTATAAC5200.015.6538461
CAAGACG10200.015.2352944
TCCGATA4600.014.8804348
GACGGAC9350.014.8395737
CCGATAA4500.014.89
CGCGATA750.004106675314.814
TATACTG6250.014.85
GTACCGA1001.0943964E-414.7999996
CGTATAC1001.0943964E-414.7999993
ACGGACC9650.014.7616598
AGACGGA10700.014.5233646
ATACCGT6850.014.31386856
TAATACT9350.014.245994
ATAACGA5000.014.06000112
TAACGAA4800.013.87500113
Produced by FastQC (version 0.11.5)