FastQCFastQC Report
Thu 28 Apr 2016
ERR1042471.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042471.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2073858
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT160650.7746432012220702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT142470.6869804972182281No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73250.35320643939941887No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69750.3363296812028596No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC61110.29466819811192474No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA49920.24071079119206812No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG32290.15570014919054245No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG31520.15198726238729943No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG30610.14759930525619402No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT29660.1430184708885565No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG26520.1278776078207862No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT25270.12185019417915788No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22400.10801125245797928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA500.00703674418.55
TATAACG1950.018.0256422
TATTCCG752.0682772E-417.2666665
TATACTG3800.017.0394745
CGAACGA2400.016.95833216
AAGACGG7550.016.4172175
ATGCGAA700.002593437215.8571426
CCGATAA2700.015.7592589
ACGAACG2600.015.65384615
ACGGACC7200.015.4166668
CGCAAGA7500.015.2933342
ATTAGAC2300.015.2826093
TAACGAA2800.015.19642813
GTATAAC2800.015.1964281
ATAACGA2800.015.19642812
GACGGAC7450.015.1476517
AGGTCGC2700.015.07407335
CGGACCA7350.014.850349
CCGATAC750.00410588714.83
GTAATAC2650.014.66037753
Produced by FastQC (version 0.11.5)